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Aggressiveness and Molecular Variability
of Fusarium solani f. sp. glycines

Sudden death syndrome of soybean is caused by Fusarium solani f. sp. glycines (FSG). A total of 78 FSG isolates was collected from different geographic locations. These isolates were used to inoculate a susceptible soybean cultivar, Great Lakes 3202, in a growth chamber using FSG-infested sorghum, which was placed below seeds at planting. Foliar symptoms, root and root lesion lengths, and shoot and root dry weights were recorded 21 days after planting.

There was a significant difference (P < 0.05) among isolates for foliar disease severity ranging from 2 to 5, using a 1 to 5 disease severity scale where 1 = no disease and 5 = severely infected or dead plants. Root lesion lengths varied from 12 to 110 mm. Significant differences also were observed in shoot and root dry weights. Amplified fragment length polymorphism (AFLP) analysis detected DNA polymorphisms using eight combinations of fluorescence-labeled primer sets in the selective amplification. In this study, FSG isolates varied in aggressiveness on soybean and in DNA polymorphisms based on AFLP analysis.

Shuxian Li, Principal Research Specialist
Department of Crop Sciences
(217) 244-9293; sli1@illinois.edu

Glen Hartman, USDA-ARS and Associate Professor
Department of Crop Sciences and USDA–ARS
(217) 244-3258; ghartman@illinois.edu

Wayne Pedersen, Associate Professor
Department of Crop Sciences
(217) 333-3847; wpederse@illinois.edu

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